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Guide to Techniques in Mouse Development, Part A comprehensively covers new technologies and methodologies that have appeared for the study of mouse development. Update of volume 225 of Methods in Enzymology, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993Covers new technologies and methodologies, including: new techniques for the cryopreservation of gametes and embryosproduction of transgenic and (knockout) animals (use of ES cells)generation of conditional/inducible mutant animalsuse of gene-trap mutagenesisanalysis of allele-specific expresionuse of new reporter constructshumanizing of transgenic animalstranscript profiling of mouse developmentimaging of mouse developmentrederivation of animals and use of mouse genomics
