This volume discusses basic and advanced techniques to study macrophages and their unique properties. The chapters in this book cover numerous topics such as in vitro culture models for murine and human macrophages; isolation of resident macrophages from several tissues; functional analyses of macrophages; and transgenic models of macrophage depletion and macrophage targeting. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive,Macrophages: Methods and Protocolsis a valuable resource for researchers who are interested in studying macrophages on an experimental level.
The chaptersA Simple Multi-Step Protocol for Differentiating Human Induced Pluripotent Stem Cells into Functional MacrophagesandIsolation and Phenotyping of Adult Mouse Microglial Cellsare open access under a CC BY 4.0 license via link.springer.com.
