This volume explores protocols for identifying mutant mice and characterizing parts of their anatomical, functional, cellular, and molecular phenotypes. The chapters in this book look at anatomical and functional phenotyping using quantitative imaging, isolation of specific embryonic cell types for cell culture, analysis of gene expression, and how to define chromatin structure. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative,Mouse Embryogenesis: Methods and Protocolsis a valuable resource for experimentalists interested in discovering new aspects of embryogenesis control, organ function, and the origin of disease.