This book details methods on various aspects of the very final stages of mouse oocyte development. Chapters guide the reader throughin vitro growth of follicles, production of a fully-grown competent mouse oocyte, meiosis resumption, analysis of spindle assembly and chromosome segregation, analysis of the oocyte and early embryo transcriptome. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge,Mouse Oocyte Development: Methods and Protocolsaims to help scientists with the set up both live and fix experiments required to study the process of oocyte meiotic maturation.
