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Skeletal Development and Repair: Methods and Protocolsis a compilation of a variety of skeletal research protocols utilizing the laboratory mouse as the platform for surgical manipulation and/or transplantation as well as the source of tissues and cells forin vitroculture and analyses. Chapters are written by experts in the field and cover topics including surgical, transplantation and organ culture methods that permit analyses of skeletal tissues undergoing repairin vivoand permits analyses of cellular interactionsex vivo, histological and molecular techniques developed to study gene and protein expression in whole embryos, skeletal tissues and tissue sections andin vitroprimary cell culture protocols designed to assay gene function in specific cell populations. Written in the successfulMethods in Molecular Biologyseries format chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible,Skeletal Development and Repair: Methods and Protocolsis a comprehensive laboratory manual for all levels of basic research scientists working in the broad fields of skeletal development and skeletal repair research.
